Nutritional growth characteristics

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فهرست عناوین اصلی در این پاورپوینت

● Learning objectives
● Cultivation of bacteria
● Why cultivate bacteria?
● Nutritional growth characteristics
● Growth requirements
● Temperature
● pH
● Osmotic pressure
● Moisture and desiccation
● Carbon
● Oxygen
● Types of bacterial culture media
● Selective & differential media
● Anaerobic growth
● Capnophiles
● Culture methods
● Pure cultures, plate or any of the others
● Bacteria grow by binary fission
● Phases of bacterial growth
● Inside the tissue: interpretation
of the bacterial growth curve
● Rate of bacterial death
● Bactericidal versus bacteriostatic
● Measurement of cell growth
● Rapid cultivation & automation

نوع زبان: انگلیسی حجم: 0.27 مگا بایت
نوع فایل: اسلاید پاورپوینت تعداد اسلایدها: 29 صفحه
سطح مطلب: نامشخص پسوند فایل: ppt
گروه موضوعی: زمان استخراج مطلب: 2019/06/07 07:51:31

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عبارات مهم استفاده شده در این مطلب

عبارات مهم استفاده شده در این مطلب

bacterium, laboratory, training, field, epidemiologist, growth, culture, grow, media, bacterial, oxygen, anaerobic,

توجه: این مطلب در تاریخ 2019/06/07 07:51:31 به صورت خودکار از فضای وب آشکار توسط موتور جستجوی پاورپوینت جمع آوری شده است و در صورت اعلام عدم رضایت تهیه کننده ی آن، طبق قوانین سایت از روی وب گاه حذف خواهد شد. این مطلب از وب سایت زیر استخراج شده است و مسئولیت انتشار آن با منبع اصلی است.

https://www.who.int/ihr/lyon/surveillance/methods/ihr_l8baculture_en.ppt

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عبارات پرتکرار و مهم در این اسلاید عبارتند از: bacterium, laboratory, training, field, epidemiologist, growth, culture, grow, media, bacterial, oxygen, anaerobic,

مشاهده محتوای متنیِ این اسلاید ppt

مشاهده محتوای متنیِ این اسلاید ppt

laboratory training for field epidemiologists cultivation of bacteria investigation strategies and methods may ۲ ۷ laboratory training for field epidemiologists learning objectives at the end of the presentation participants should understand the principles of cultivating bacteria understand the methods and problems faced when cultivating bacteria laboratory training for field epidemiologists cultivation of bacteria the process of growing microorganisms in culture by taking bacteria from an infection site by specimen collection in vivo growing bacteria in the artificial environment of the laboratory in vitro laboratory training for field epidemiologists why cultivate bacteria obtain definitive identification and characterization grow and isolate all bacteria present in an infection determine which bacteria is most likely causing infection determine which bacteria is likely a contaminant or colonizer laboratory training for field epidemiologists why cultivate bacteria obtain sufficient growth of clinically relevant bacteria to test antimicrobial susceptibility measure response to treatment characterize the agent bank strain for future use including vaccine development laboratory training for field epidemiologists nutritional growth characteristics autotrophs lithotrophs able to utilize simple inorganic compounds co۲ as carbon source ammonium salts as nitrogen source include photoptrophs photosynthesis and chemolithotrophs oxidation of inorganic compounds heterotrophs bacteria in human body unable to synthesize own metabolites depend on preformed organic compounds nutritional needs are variable laboratory training for field epidemiologists growth requirements physical temperature ph osmotic pressure moisture desiccation chemical carbon source nitrogen sulfur phosphorus oxygen rate of growth temperature in ºc ۱ ۱ ۲ ۳ ۴ ۵ ۶ ۷ ۸ ۹ ۱ ۱۱ psychrophiles psychrotrophs mesophiles thermophiles hyperthermophiles laboratory training for field epidemiologists temperature psychrophiles cold loving true psychrophiles optimum growth at ۱۵ ºc psychrotophs optimum growth at ۲ ۳ ºc mesophiles moderate temperature loving thermophiles heat loving hyperthermophiles tolerate extreme temperatures most pathogenic bacteria are mesophiles and grow optimally at ۳۷ c human body temperature laboratory training for field epidemiologists ph most medically important bacteria grow at neutral or slightly alkaline ph ۷.۲ to ۷.۶ very few bacteria grow below ph ۴ lactobacilli grow in acidic ph cholera vibrio grow in alkaline ph growth media includes chemical buffers to prevent acid production foods are preserved by acids produced by bacterial fermentation laboratory training for field epidemiologists osmotic pressure high osmotic pressure hypertonic removes water causing plasmolysis – inhibits growth i.e. salt as preservative low osmotic pressures hypotonic cause water to enter and can cause lysis bacteria are more tolerant to osmotic variations because of the mechanical strength of the cell wall nacl .۸۵ nacl ۱ h۲o plasma membrane plasma membrane cell wall graphic selfprepared laboratory training for field epidemiologists moisture and desiccation moisture is essential ۸ body weight is water effect of drying varies by organism t pallidum gonococcus are very susceptible tubercle bacilli staphylococci may survive for weeks bacterial spores survive several years lyophilization freeze dry process that protects bacteria laboratory training for field epidemiologists carbon chemo and photo autotrophs fix co۲ chemoheterotrophs obtain energy from organic compounds laboratory training for field epidemiologists oxygen obligate aerobes only aerobic growth oxygen required facultative anaerobes most human pathogens greater growth in presence of oxygen obligate anaerobes only anaerobic growth cease with oxygen aerotolerant anaerobes e.g. c. perfringens only anaerobic growth continues with oxygen microaerophiles e.g. m. tuberculosis only aerobic growth with little oxygen bacterial growth in solid growth medium graphic selfprepared laboratory training for field epidemiologists types of bacterial culture media solid semisolid liquid biphasic simple media special media enriched selective enrichment indicator differential transport synthetic media aerobic and anaerobic media cell culture for obligate intracellular bacteria e.g. chlamydia spp biphasic culture medium agar slope solid medium broth liquid medium laboratory training for field epidemiologists selective differential media selective bismuth sulfite for salmonella typhi inhibits gram positive and most gram negative intestinal bacteria differential blood agar plates for streptococcus pyogenes selective differential mannitol salt agar for staphylococcus aureus type of hemolysis reaction aids identification of s. pyogenes laboratory training for field epidemiologists anaerobic growth reducing media containing thioglycolate to deplete oxygen cooked meat broth anaerobic jar anaerobic chamber anaerobic bags pouch laboratory training for field epidemiologists capnophiles capnophiles require high concentration of co e.g. brucella abortus petri plates candle tubes with liquid media glass jar petri plates gas generator gas generator a. glass jar b. co۲ generating package laboratory training for field epidemiologists culture methods streak culture isolation of bacteria in pure culture from clinical specimen lawn culture antimicrobial susceptibility testing disc diffusion bacteriophage typing liquid cultures stroke culture to obtain pure growth for slide agglutination biochemical tests stab culture maintenance of stock cultures pour plate culture quantification of bacteria in liquid cultures urine sample laboratory training for field epidemiologists culture methods continuous bacterial culture maintain a bacterial population at a constant density keeping a constant environment oxygen nutrient etc. imitates the growth in the environment a chemostat from chemical environment is static is a device used in microbiology for growing and harvesting microbes. it consists of two primary parts a nutrient reservoir and a growth chamber. the most important feature of a chemostat is that all fermentation parameters growth chamber volume dissolved oxygen nutrient concentrations ph cell density etc remain constant throughout the experiment. from wikipedia laboratory training for field epidemiologists pure cultures plate or any of the others in theory each colony ~۱ ۷ bacteria arises from a single bacterium deposited on the surface of the petri dish each colony consists of a pure clone of cells best obtained on solid media less sure in liquid media photos public domain laboratory training for field epidemiologists bacteria grow by binary fission in rich broth the number of bacteria doubles every ۳ minutes generation time if start with ۳ people in the room in ۳ minutes we would have ۶ people in ۶ minutes we would have ۱۲ very uncomfortable in ۹ minutes we would have ۲۴ suffocation laboratory training for field epidemiologists phases of bacterial growth laboratory training for field epidemiologists inside the tissue interpretation of the bacterial growth curve explosiveness of exponential growth short generation time small number of bacteria initiate a dangerous illness e.g. acute meningococcal meningitis . long generation time tuberculosis bacillus causes chronic illness inside body tissues bacteria are stressed bacterial populations are rarely fully viable may cease growth but continue synthetic activities to meet adaptive stress non growing bacteria can also be harmful immunogenic production of toxins starts or accelerates during stationery phase sporulation can release toxins laboratory training for field epidemiologists rate of bacterial death death is exponential after ۱’ – ۱ …

کلمات کلیدی پرکاربرد در این اسلاید پاورپوینت: bacterium, laboratory, training, field, epidemiologist, growth, culture, grow, media, bacterial, oxygen, anaerobic,

این فایل پاورپوینت شامل 29 اسلاید و به زبان انگلیسی و حجم آن 0.27 مگا بایت است. نوع قالب فایل ppt بوده که با این لینک قابل دانلود است. این مطلب برگرفته از سایت زیر است و مسئولیت انتشار آن با منبع اصلی می باشد که در تاریخ 2019/06/07 07:51:31 استخراج شده است.

https://www.who.int/ihr/lyon/surveillance/methods/ihr_l8baculture_en.ppt

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